Ratio of genotype calls within 1AL, 4BS and you will 5A QTL countries into the several parents and you can 81 DH traces
graminearum infection at dos dpi, as estimated of the four actions revealed a lot more than (%Fg reads, Fg-GAPDH, Fg-?TUB and you can Don) (Fig. 1). About three QTL have been known (p ? 0.05, one thousand permutations): a city for the 1AL (height in the cM, LOD support interval ranging from and you may cM) is actually of the Don account; places toward chromosome 4BS (top at the cM, LOD assistance interval anywhere between and you may cM) and you may 5A (peak in the cM, LOD help period between and you will cM) was in fact of F. graminearum levels (%Fg reads).
QTL LOD scores along wheat chromosomes for a set of 12,325 polymorphic markers. Stars indicate significant peaks (1AL, 4BS and 5A) above thresholds determined using 1000 permutations and p < =0.05
The analysis away from genotypes telephone call frequencies contained in this QTL 1AL, 4BS, and 5A for the DH contours purchased based on phenotypes (%Fg reads and you will Wear) (Fig. 2) indicated that DH contours that have straight down issues account (deep blue) got genotype AA to your QTL 1AL and you will 4BS, and you may genotype BB on the QTL 5A, hence DH contours which have higher problems account (yellow) had the reverse development, and that indicates that alleles regarding the quicker issues account were passed on out-of Wuhan step one to own QTL 1AL and you can 4BS and you will handed down regarding Nyubai to own QTL 5A.
Phone calls boosting conditional genotype likelihood from a hidden Markov design had been accustomed assess size (vertical axis) whereas AA (Wuhan step one) and you will BB (Nyubai) portray a hundred% of associated genotype for the given QTL part and you can Ab means 50% A and you will 50% B. Continue reading “QTL mapping was performed with the quantity of F”